Part:BBa_K2924023:Design

accB

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 432
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

The gene from E. Coli K12 was cloned into pet21a in an operon-like manner to ensure transcription of all genes of the complex at the same level and from the same vector.

Source

The gene was cloned from the genome of E. coli K12